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American Society of Microbiology ABMM Sample Questions:

1. For epidemiological tracking and outbreak investigations of Clostridioides difficile infection (CDI), which molecular typing method based on PCR amplification targeting repetitive elements within the pathogenicity locus (PaLoc) has historically been widely used and designated by specific nomenclature (e.g., NAP1/BI
/027)?

A) Whole-genome sequencing (WGS) SNP analysis
B) Multilocus sequence typing (MLST)
C) PCR Ribotyping
D) Pulsed-field gel electrophoresis (PFGE)

2. A clinical microbiology laboratory encounters a Gram-negative bacillus isolated from a bloodstream infection. The isolate exhibits resistance to multiple antibiotics, including carbapenems. Further phenotypic testing reveals the production of a metallo-beta-lactamase (MBL). Which of the following antibiotic combinations might still retain activity against this isolate?

A) Piperacillin-tazobactam
B) Ceftazidime-avibactam
C) Aztreonam-avibactam
D) Aztreonam

3. A patient with a history of neutropenia following chemotherapy develops a rapidly progressive pneumonia.
Bronchoalveolar lavage fluid reveals septate hyphae with acute angle (45-degree) branching. The MOST likely diagnosis is invasive pulmonary aspergillosis caused by:

A) Mucor species
B) Fusarium species
C) Rhizopus species
D) Aspergillus fumigatus

4. During a primary infection with Epstein-Barr virus (EBV), the virus establishes latency in B lymphocytes.
The transition from lytic to latent infection is tightly regulated by viral gene expression. Which of the following viral proteins is crucial for the establishment and maintenance of EBV latency and is a key target in EBV-associated lymphomas?

A) Epstein-Barr nuclear antigen 1 (EBNA1)
B) Glycoprotein gp350
C) Viral capsid antigen (VCA)
D) Early antigen (EA)

5. Which pattern on a Kirby-Bauer disk diffusion plate, showing resistance of Staphylococcus aureus to erythromycin but susceptibility to clindamycin with a flattening of the clindamycin zone adjacent to the erythromycin disk (D-zone test positive), indicates inducible clindamycin resistance?

A) Indicates the strain possesses the msrA gene
B) Indicates the strain should be reported as clindamycin susceptible
C) Indicates the strain is tolerant to clindamycin
D) Indicates the strain possesses an erm gene and should be reported as clindamycin resistant

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